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我們與世界各地的科學家們繼續為生命科學領域增加了新的知識
哈佛大學(美國)
Harvard University
The mutants were constructed by PCR-based site-directed mutagenesis (Muta-directTM kit, Beijing SBS Genetech Co., Ltd., China).
倫敦帝國理工大學(英國)
Imperial College London
The wild CII263–272peptide (FKGE-QGPKGE), wild HA308–317peptide (YVKQNTLKLA), altered HA308–317peptide (YAKQATLALA), and irrelevant peptide (ALALTAQKAY) were synthesized by solid-phasetechniques (SBS Genetech). The purity was > 95%.
新加坡國立大學(新加坡)
National University of Singapore
Ten peptides corresponding to the amino acids 20–39, 30–49, 42–61, 50–69, 60–79, 73–92, 88–108, 101–120, 111–130 and 124–144 of the Blo t 12.0101 protein were prepared by solid-phase synthesis with an automated peptide synthesizer, purified by high-performance liquid chromatography and (HPLC) and analysed by mass spectrometry(SBS Genetech, Beijing, China).
康奈爾大學(美國)
Cornell University
The PCR products were checked for effective amplification through electrophoresis in 1% agarose gels containing 1:20 GoldView (SBS Genetech Co., Beijing, China).
清華大學(中國)
Tsinghua University
The locked nucleic acids (LNA) targeting ZIKV sfRNA were chemically synthesized and HPLC purified by Beijing SBS Genetech Co., Ltd.
密歇根大學(美國)
University of Michigan
These oligonucleotides were designed to amplify a 250-bp segment of GAPDH that serves as an internal standard. The primers were purchased from SBS Genetech Co. Ltd, Beijing, China.
北京大學(中國)
Peking University
A Site‐Directed Mutagenesis Kit (SBS Genetech Co., Ltd) was then used to mutate the miR‐375 binding site in the 3′‐UTR of IGFBP3 and named as IGFBP3‐mutant‐type (MT) luciferase reporter plasmid.
約翰霍普金斯大學(美國)
Johns Hopkins University
Fluorescein isothiocyanate (FITC)‐labeled peptides (N‐terminal) usedthroughout this manuscript were synthesized from SBS Genetech(Beijing, China), and purified using reversed phase analytical high‐performance liquid chromatography (>99% purity).
香港大學(中國)
The University of Hong Kong
The PCR reaction mixture consisted of 2 μL DNA (about 15 ng), 2.5 μL of 10 × PCR buffer, 1.5 μL of 25 mmol/L MgCl2, 1.5 μL of 2.5 mmol/L dNTPs, 1.5 U of Taq DNA polymerase (SBS Genetech Co., China), 2.0 μL each of 2.5 μmol/L IT1F and IT2R primers (synthesized by SBS Genetech Co., China) in a final volume of 25 μL.
加州大學伯克利分校(美國)
University of California, Berkeley
Amplified products were run on a 2% agarose gel with GoldView (SBS Genetech Co., Ltd., Beijing) for visualization.
多倫多大學(加拿大)
University of Toronto
Point mutations of RokC114-185 were generated using the site-directed mutagenesis kit (SBS Genetech).
京都大學(日本)
Kyoto University
Genomic DNA was extracted by Genome DNA Simax Kit (Beijing SBS Genetech Co., Ltd., China).
麥吉爾大學(加拿大)
McGill University
Fluorescein isothiocyanate (FITC)‐labeled peptides (N‐terminal) used throughout this manuscript were synthesized from SBS Genetech(Beijing, China), and purified using reversed phase analytical high‐performance liquid chromatography (>99% purity).
首爾國立大學(韓國)
Seoul National University
Each 25 μL reaction mixture contained 50 ng DNA, 5 pmol of each primer, 2 μL PCR buffer [100mM Tris (pH 8.3), 500 mM KCl, 15 mM MgCl2], 250 μM of each dNTPs and 0.5-1 unit Taq DNA polymerase (SBS Genetech Co, Beijing, China).
浙江大學(中國)
Zhejiang University
Total RNA was then extracted using Redzol reagent (SBS Genetech Inc., Beijing, China)
威斯康星大學麥迪遜分校(美國)
University of Wisconsin, Madison
Due to the high content of phenol and polysaccharides in pear leaves, genomic DNA was then purified using a purification kit from SBS Genetech Co. Ltd., China.
上海交通大學(中國)
Shanghai Jiao Tong University
RNA was isolated using the Total RNA Isolation Kit (Beijing SBS Genetech Co., Ltd.) from mycelia of WT and its derivative ΔctcS mutant strains grown two days in fermentation medium.
墨爾本大學(澳大利亞)
University of Melbourne
After DNA extraction, extracts were loaded on 1% agarose gel in 0.5x Tris-Acetate-EDTA (TAE) buffer with GoodView Nucleic Acid Stain (SBS Genetech Co., Ltd., China) and were electrophoresed (100 V).
復旦大學(中國)
Fudan University
All primers were designed by Primer 5.0 software and synthesized by SBS Genetech (Beijing, China).
新南威爾士大學(澳大利亞)
The University of New South Wales
We checked PCR ampli-cons with an electrophoresis in 0.7% agarose gelsstained using GoodView (SBS Genetech, Beijing, China)for visualization in UV light.
英屬哥倫比亞大學(加拿大)
University of British Columbia
Gap26 and10panx1 were synthetized by SBS Genetech (Beijing, China).
中國科學技術大學(中國)
University of Science and Technology of China
The mutant luciferase reporter con-structs of HIF‐1 were constructed using Site‐DirectedMutagenesis Kit (SBS Genetech, Beijing, China).
昆士蘭大學(澳大利亞)
University of Queensland
Reverse-transcription of total RNA into the first-strand cDNA was performed by an RT-PreMix kit (SBS Genetech) and a universal dT3AP [oligo(dT)-containing adaptor primer], which were directly used as templates for RACE (3 rapid amplification of cDNA ends) with primers MeuICK-F1 and 3AP (Figure 1A).
蒙納士大學(澳大利亞)
Monash University
Desired mutations were introduced to the N-terminal double c-Myc–labeled human GLP-1R gene in pDONR201 (Invitrogen) via the Muta-directTM kit (Beijing SBS GenetechCo., Ltd., China)
武漢大學(中國)
Wuhan University
Fluorescein isothiocyanate (FITC)‐labeled peptides (N‐terminal) used throughout this manuscript were synthesized from SBS Genetech(Beijing, China), and purified using reversed phase analytical high‐performance liquid chromatography (>99% purity).
加州大學圣迭戈分校(美國)
University of California, San Diego
To make 5D, 5D-L, 5D-a, ASF-L and Tra2b-L series of constructs, sense and antisense oligos containing a full or truncated XhoI and EcoRI restriction sites were synthesized (SBS Genetech, Beijing) and diluted in the linker buffer (50 mM Tris–HCl pH 8.0, 100 mM NaCl, 1 mM EDTA) at a final concentration of 100 nM.
蘇黎世大學(瑞士)
University of Zurich
To make 5D, 5D-L, 5D-a, ASF-L and Tra2b-L series of constructs, sense and antisense oligos containing a full or truncated XhoI and EcoRI restriction sites were synthesized (SBS Genetech, Beijing) and diluted in the linker buffer (50 mM Tris–HCl pH 8.0, 100 mM NaCl, 1 mM EDTA) at a final concentration of 100 nM.
馬來亞大學(馬來西亞)
University of Malaya
All the oligonucleotide probes and target were procured from SBS Genetech Co., Ltd (Beijing, PRC)
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